The amicon~R Pro sysrem - a centrifugal device capable of performing all steps in the protein purification workflow

摘要

Traditional protein purification is a long process with many steps utilizing multiple devices, often resulting in protein degradation and loss. The Amicon~R Pro device streamlines the affinity purification process by providing a single adaptable centrifugation unit capable of performing all steps in the affinity purification process. The device combines affinity-based spin column purification with downstream sample concentration and buffer exchange, eliminating the need for multiple sample transfers, thereby minimizing protein loss. The results presented in this work indicate that purification of His-tagged protein using the Ami-con~R Pro device is faster, easier, and provides better yields than other traditional methods (eg. spin-column and slurry method).