Two versatile shuttle vectors for Thermus thermophilus-Escherichia coli containing multiple cloning sites, laczα gene and kanamycin or hygromycin resistance marker

摘要

Two versatile shuttle vectors for . Thermus thermophilus and . Escherichia coli were developed on the basis of the . T. thermophilus cryptic plasmid pTT8 and . E. coli vector pUC13. These shuttle vectors, pTRK1T and pTRH1T, carry a gene encoding a protein homologous to replication protein derived from pTT8, a replicon for . E. coli, new multiple cloning sites and a . laczα gene from . E. coli vector pUC13, and also have a gene encoding a thermostable protein that confers resistance to kanamycin or hygromycin, which can be used as a selection marker in . T. thermophilus. These shuttle vectors are useful to develop enzymes and proteins of biotechnological interest. We also constructed a plasmid, pUC13T, which carries the same multiple cloning sites of pTRK1T and pTRH1T. These vectors should facilitate cloning procedures both in . E. coli and . T. thermophilus.