Localized Surface Plasmon Resonance-Based Fiber Optic U-Shaped Biosensor for the Detection of Blood Glucose

摘要

In the present study, we report the first fiber optic glucose sensor utilizing localized surface plasmon resonance of metal nanoparticles. The fiber was bent in the form of a U-shaped probe for point detection and sensitivity enhancement. The probe was prepared by first attaching gold nanoparticles on the optical fiber core and then immobilizing glucose oxidase over it. The sensor operates in the intensity modulation scheme in which the absorbance is measured with respect to the changes in the glucose concentration. The presence of glucose in the vicinity of the sensing region changes the refractive index of the film due to the chemical reactions with glucose oxidase. The absorbance of the metal nanoparticle changes significantly due to local refractive index change. The fiber optic Ushaped probes of different bending radii were fabricated and it has been found that the probe with bending radius around 0.982 mm possesses the maximum sensitivity. The response of the sensor is fast and requires very small volume of sensing sample (~150 μl) which makes it more suitable for commercialization and better than present commercial sensors, which require about 1.5 ml of blood for the detection of glucose.