Cartilage adhesive and mechanical properties of enzymatically crosslinked polysaccharide tyramine conjugate hydrogels?

摘要

Using a home-built tensile tester, adhesion and mechanical properties of injectable enzymatically crosslinkable hydrogels were determined by placing the hydrogels in between cartilage surfaces. Dextran-tyramine (Dex-TA) and hyaluronic acid-tyramine (HA-TA) conjugates as well as a 50/50 composite material of these polysaccharide conjugates were tested. To integrate the injectable hydrogels with the cartilage tissue, pretreatment of the tissue with a Dex-TA conjugate solution strongly improved the adhesion. Only failure of the crosslinked hydrogelwas observed and not at the hydrogel-tissue interface. Moduli of a Dex-TA hydrogel are higher than those of a HA-TA hydrogel, whereas the ultimate strain of the HA-TA hydrogel was at least three times higher. The Dex-TA/HA-TA hydrogel has similar storage and elastic moduli as the Dex-TA gel and also an ultimate strain of ～30%, similarly as found for the HA-TA gel. The controlled biodegradability and gelation time of the Dex-TA/HA-TA hydrogel, the developed method for strong tissue adhesion of the gel particularly in comparison with fibrin glue, makes this material applicable as an injectable hydrogel for tissue regeneration applications.