Supercritical CO₂ assisted extraction and LC-MS identification of picroside I and picroside II from Picrorhiza kurroa.

摘要

Introduction - Picroside I and picroside II have been studied intensively because of their pharmacological actions and clinical applications. Numerous methods have been reported for extracting picroside I and picroside II from Picrorrhiza. kurroa rhizomes. This is the first report of picroside I and picroside II extraction using the supercritical carbon dioxide assisted extraction technique. Objective - To develop supercritical carbon dioxide assisted extraction and LC-MS identification of picroside I and picroside II from the Picrorrhiza kurroa Royle rhizomes. Methodology - Surface response methodology based on 33 fractional factorial design was used to extract picroside I and picroside II from P. kurroa rhizomes. The effects of various process factors, namely temperature (40-80 degrees C), pressure (25-35 MPa) and co-solvent (methanol) concentration (0-10% v/v) on extraction yield of the two compounds were evaluated. The picroside I and picroside II contents were determined using validated LC-MS methodology. Results - The maximum yield of picroside I (32.502+or-1.131 mg/g) and picroside II (9.717+or-0.382 mg/g) was obtained at the 10% v/v co-solvent concentration, 40 degrees C temperature and 30 MPa pressure. The conventional Soxhlet assisted methanol extract of P. kurroa powder resulted in 36.743+or-1.75 and 11.251+or-0.54 mg/g yield of picroside I and picroside II, respectively. Conclusion - Variation of concentration and extraction time showed a significant effect on the picroside I and picroside II yield. Supercritical carbon dioxide assisted extraction using methanol as a co-solvent is an efficient and environmentally sustainable method for extracting picroside I and picroside II from P. kurroa rhizomes.Digital Object Identifier http://dx.doi.org/10.1002/pca.2383