Standardisation of Gymnema sylvestre R. Br. with reference to gymnemagenin by high-performance thin-layer chromatography

摘要

A simple and reproducible HPTLC method for the determination of gymnemagenin (1) in Gymnema sylvestre has been developed. Components were separated on pre-coated silica gel 60 F-254 plates with chloroform:methanol (9:1) and scanned using a densitometric scanner in the UV reflectance mode at 290 nm. Linearity of determination of 1 was observed in the range 4-10 mug. The average percentage recovery of 1 from an extract was 99.09 +/- 0.29, and the content of 1 in leaves of the title plant was 1.61% (dry weight).