Glutaraldehyde Crosslinked Gelatin/hydroxyapatite Nanocomposite Scaffold, Engineered via Compound Techniques

摘要

In this study, a scaffold was designed to be used in bone tissue repair and the effect of glutaraldehyde (GA) concentration as crosslinking agent was investigated. To mimic the mineral and organic component of natural bone, hydroxyapatite (HAp) and gelatin (GEL) were used as the main components of this composite. Nanopowders of HAp were synthesized and also used together with GEL to engineer a three-dimensional nanocomposite scaffold. The results show that GEL/ HAp nanocomposite is porous with three-dimensional interconnected structure, pore sizes ranging from 300 to 500 μm, and about 85% porosity. In addition, increasing GA concentration provokes the enhancement of compressive strength until 1 w/v% GA solution followed by a reduction to 2.5%, whereas it causes work fracture to decrease. It was concluded that optimum concentration for crosslinking GEL matrix for this purpose is 1 w/v% GA solution. A specific combination of commonly used techniques applied to engineer a scaffold with almost ideal properties intended for the bone tissue engineering is introduced. In addition, scaffolds that are prepared via this compound process has the potential to be used in the solid free form applications and so being formed in any dimension and geometry relevant to the defect size and Shape.