Influence of nonsteroidal anti-inflammatory drugs on progesterone production by cultured bovine luteal cells.

摘要

The objective of the present study was to determine the influence of nonsteroidal anti-inflammatory drugs (NSAIDs) representing different chemical groups on progesterone (P₄) production by cultured bovine steroidogenic luteal cells. The cells were enzymatically isolated from corpora lutea collected on days 8-12 of the estrous cycle. After 24 h preincubation they were incubated for 24 h with medium only (control) or stimulated with bovine luteinizing hormone - LH (100 ng/ml; positive control) or increasing concentrations (10-8 to 10-4 M) of acetylsalicylic acid, indomethacin, ibuprofen, naproxen, piroxicam, phenylbutazone, dipyrone or nimesulide. Concentrations of P₄ in the culture media were determined by enzyme immunoassay. LH significantly increased P₄ secretion, while acetylsalicylic acid and indomethacin did not affect the production of this hormone. A significant increase in P₄ secretion was observed after administration of dipyrone at all concentrations, piroxicam at concentrations of 10-8, 10-7 and 10-5 M, phenylbutazone and naproxen at concentrations of 10-7 and 10-6 M and ibuprofen at concentrations of 10-5 and 10-4 M. Nimesulide did not affect P₄ production at concentrations of 10-8-10-5 M, while at a concentration of 10-4 M it inhibited P₄ secretion. The results obtained indicate that NSAIDs may change the production of P₄ in bovine luteal cells, however, these changes are dependent on the substance used.