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首页> 外文期刊>Polish Journal of Microbiology >Antibiotic Susceptibility and Genotype Patterns of Escherichia coli, Klebsiella pneuomoniae and Pseudomonas aeruginosa Isolated from Urinary Tract Infected Patients
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Antibiotic Susceptibility and Genotype Patterns of Escherichia coli, Klebsiella pneuomoniae and Pseudomonas aeruginosa Isolated from Urinary Tract Infected Patients

机译:尿路感染患者中分离的大肠杆菌,肺炎克雷伯菌和铜绿假单胞菌的抗生素敏感性和基因型

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摘要

Thirty nine isolates of Escherichia coli, twenty two isolates of Klebsiella pneumoniae and sixteen isolates of Pseudomonas aeruginosa isolated from urinary tract infected patients were analyzed by antimicrobial susceptibility typing and random amplified polymorphic DNA (RAPD)-PCR. Antibiotic susceptibility testing was carried out by microdilution and E Test methods. From the antibiotic susceptibility, ten patterns were recorded (four for E. coli, three for K. pneumoniae and three for P aeruginosa respectively). Furthermore, genotyping showed seventeen RAPD patterns (seven for E. coli, five for K. pneumoniae and five for P aeruginosa respectively). In this study, differentiation of strains of E. coli, K. pnewnoniae and P aeruginosa from nosocomial infection was possible with the use of RAPD.
机译:通过抗生素敏感性分型和随机扩增多态性DNA(RAPD)-PCR分析了从尿路感染患者中分离出的39株大肠杆菌,22株肺炎克雷伯菌和13株铜绿假单胞菌。抗生素敏感性试验通过微量稀释和E检验方法进行。根据抗生素的敏感性,记录了十种模式(分别为四种大肠杆菌,三种肺炎克雷伯菌和三种铜绿假单胞菌)。此外,基因分型显示了17种RAPD模式(大肠杆菌为7种,肺炎克雷伯菌为5种,铜绿假单胞菌为5种)。在这项研究中,使用RAPD可以使大肠杆菌,肺炎克雷伯氏菌和铜绿假单胞菌菌株从医院感染中分化出来。

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