Genetic variations of gliadin and HMW-glutenin subunits in Triticum turanicum Jakubz.

摘要

To detect the genetic variations of gliadin and high molecular weight (HMW) glutenin subunits in Triticum turanicum Jakubz., 87 accessions from 24 countries were analysed using acid polyacryl-amide gel electrophoresis (A-PAGE) and sodium dodecyl sulphate poly-acrylamide gel electrophoresis (SDS-PAGE), and 72 gliadin patterns were identified. The genetic similarity (GS) of gliadin ranged from 0.240 to 1.000, with a mean of 0.693. Clustering analysis indicated that the genetic variation of gliadin in T. turanicum was associated with its geographical distribution. Two and five HMW-GS alleles were identified at Glu-Al and Glu-Bl, respectively. For the Glu-Bl locus, subunits 13+16, associated with good bread-making quality, were the most frequent subunits in T. turanicum, whereas it was rare or appeared with a low frequency in bread wheat and durum wheat. The variation of Glu-Al was similar to bread wheat and durum wheat, and subunit null was the most common allele. These results suggested that gliadinand HMW-glutenin subunits could represent useful markers for the evaluation of genetic variation in T. turanicum and subsequently for their future utilization.