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Romanian wheat (Triticum aestivum L.) landraces characterized by seed storageprotein electrophoresis

机译:罗马尼亚小麦(Triticum aestivum L.)地方品种,其特征在于种子贮藏蛋白电泳

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Forty Romanian wheat landraces, stored in the active collection of the Suceava Genebank, were characterized by means of glutenin analyses. Their HMW-GS patterns, homogeneity and heterogeneity, and Glu-score were determined and characterized by SDS-PAGE. Seed storage protein patterns analysed in this study indicate a relatively high level of protein heterogeneity. Only five of the accessions analysed were found to be homogeneous: 87.5% were heterogeneous, with from 2 to 14 different protein patterns.In total 202 different protein phenotypes (lines, biotypes) were detected. Together 13 HMW-GS alleles were detected at all three Glu-1 loci. The most frequent, at relevant loci, were alleles null, 7+9 and 5+10. More than 10% of protein, phenotypes reached a maximal value Glu-score.
机译:利用谷蛋白分析对40个罗马尼亚小麦地方品种(存储在Suceava种质库的活跃馆藏中)进行了表征。通过SDS-PAGE确定并表征了它们的HMW-GS模式,同质性和异质性以及Glu分数。在这项研究中分析的种子贮藏蛋白质模式表明蛋白质异质性相对较高。发现只有五种被分析的材料是同质的:87.5%是异质的,具有2至14种不同的蛋白质模式。总共检测到202种不同的蛋白质表型(品系,生物型)。在所有三个Glu-1基因座上共检测到13个HMW-GS等位基因。在相关基因座上最常见的是等位基因无效,7 + 9和5 + 10。超过10%的蛋白质表型达到最大值Glu评分。

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