Production of Populus euphratica Oliv. X P. alba L. hybrid poplars through ovary and ovule cultures

摘要

Ovary and ovule embryo culture were used to produce hybrid poplar plants of Populus euphratica and P. alba. Developed ovaries and ovules were isolated from P. euphratica female branches which were then pollinated with P. alba pollen grains by usingeither the bottle grafting, mature tree, or twig and pot technique. The isolated ovaries and ovules of P. euphratica were then transferred to growth-regulator tree 50 percent. Murashige and Skoog (MS) agar medium for embryo germination. Only 45-day-old P. euphratica ovary and ovule embryos produced plantlets. Because of the long period required for embryo development when P. euphratica is used as a maternal plant, artificial pollination using bottle grafting and the twig and pot pollination system, as well as 10 to 21-day-old ovary and ovule embryos of P. euphratica, did not produce hybrid plants. A maximum of two to three plantlets was observed in P. euphratica X P. alba ovary culture. The efficiency of pollinated ovaries to produce plantlets was 67 percent, whereas 90 percent of the pollinated P. euphratica ovules successfully produced plantlets. Before being transferred to potting soil, plantlets were cultured in the same medium within jars. Thirteen P. euphratica X P. alba hybrid plants were acclimatized in a greenhouse and transferred to the field.