首页> 外文期刊>Polish Journal of Food and Nutrition Sciences >COMPARATIVE STUDIES ON IDENTIFICATION OFLISTERIA MONOCYTOGENES STRAINS PERFORMED USING A COMMERCIAL API(R)LISTERIA KIT AND PCR TECHNIQUE
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COMPARATIVE STUDIES ON IDENTIFICATION OFLISTERIA MONOCYTOGENES STRAINS PERFORMED USING A COMMERCIAL API(R)LISTERIA KIT AND PCR TECHNIQUE

机译:商业李斯特试剂盒和PCR技术鉴定李斯特菌单核细胞增生李斯特菌的比较研究

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摘要

Two advanced methods for identification of Listeria monocytogenes strains: a commercial miniaturised biochemical test - API(r)LISTERIA (bioMerieux) and polymerase chain reaction (PCR) adapted to the in vitro amplification of the lap gene fragment (453 bp), were used in comparative studies. A total of 63 strains suspected, based on morphological criteria, to belong to L, monocytogenes species were subjected to both identification procedures, with 58 strains (92,1%) being of the river water, fish, fish products, ingredients used in fish production and the fish-processing environment origin Comparison of the results showed that 87.3% strains were recognised as L. monocytogenes with both tests and expressed haemolytic activity. The PCR enabled to classi fy 3 strains of uncertain biochemical identification. Two non-haemolytic strains were confirmed to be L, monocytogenes with both tests. In the case of the third non-haemolytic isolate, the results of both tests were contradictory. None of the analysed st rains remained unidentified. Simultaneous application of both identification methods proved to be particularly useful in microbiological diagnosis of food.
机译:用于鉴定单核细胞增生李斯特氏菌菌株的两种先进方法是:商业化的微型生化试验-API(r)LISTERIA(bioMerieux)和适于体外扩增lap基因片段(453 bp)的聚合酶链反应(PCR)。比较研究。根据形态学标准,总共怀疑63株属于L,单核细胞增生性菌种都经过了两种鉴定程序,其中有58株(92,1%)是河水,鱼,鱼产品和鱼中使用的成分生产和鱼类加工环境的起源结果比较表明,两种测试均将87.3%的菌株识别为单核细胞增生李斯特菌,并具有溶血活性。 PCR能够对3种不确定的生化鉴定菌株进行分类。两种测试均证实了两种非溶血性菌株为单核细胞增生李斯特氏菌。在第三种非溶血性分离株的情况下,两个测试的结果是矛盾的。分析的st降雨均未发现。两种鉴定方法的同时应用被证明在食品的微生物诊断中特别有用。

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