PHYLOGENETIC DIVERSITY OF BACTERIA AND ARCHAEAASSOCIATED WITH THE MARINE SPONGE PACHYCHALINA SP.

摘要

Molecular techniques wereemployed to document the microbial diversityassociated with the marine sponge Pachychalinasp. from South China Sea in March 2003. Us-ing the total microbial DNA as template, bacte-rial and archaeal 16S rDNAs were amplified byPCR with universal primers. Amplified productswere cloned, sequenced and secondarily ampli-fied by PCR. Then the secondarily amplifiedproducts were purified to be further character-ized by amplified rDNA restriction analysis (AR-DRA). According to the enzyme restriction map-ping, the apparent difference among them weredisclosed. 22 bacterial cloned partial sequenceswere acquired and most of them were related toproteobacterium. Also, 7 archaeal cloned partialsequences were acquired and a phylogenetictree was built up.Result shows the prolific bac-terial and archaeal diversity of marine spongePachychalina sp..