In vitro culture of two threatened South African medicinal trees - Ocoteabullata and Warburgia salutaris

摘要

The aim of the project was to develop micropropagation procedures for the heavily exploited and endangered South African trees black stinkwood (Ocotea bullata) and pepperbark (Warburgia salutaris) to facilitate conservation and reforestation. Both species are difficult to establish and grow in tissue culture because of their high phenolic content. A protocol for the establishment of explants in vitro was developed comprising decontamination, the application of antioxidants and cold treatment.