Improvement of Aureobasidium pullulans A.p.-3 for pullulan biosynthesis by associated mutagenesis

摘要

The strain Aureobasidium pullulans A.p.-3 was subjected to mutagenesis to improve its ability to pullulan biosynthesis. The mutagenesis of 18-h old culture was carried out in two variants using ethylen imine (EI) and ultraviolet (UV) radiation at the EI concentration and UV exposure times of 2.0 mg/mL and 1.5 man (variant I), and 2.0 mg/mL and 2.0 min (variant II). Among 240 cultures examined, the positive mutants made 26% for variant I and 13% for variant II. Two best black mutants Dy-17 and Dx-30 synthesized the polysaccharide with the yields greater than those of parent strain by 24 and 21%, respectively White mutant was also obtained, the pullulan yield for which was on the level as for strain A.p.-3, but free from melanin contaminants. Acquired characteristic of black mutants was found stable when re-examined after 3- and 6-month storage at 4 deg C. The kinetics of pullulan biosynthesis by black mutants indicated that the pullulan production was intensified beginning from the second day of culturing, as compared to strain A.p.-3.