Spectral Properties and DNA-binding Study of 6a,12a-Diazadibenzo-[a,g]fluorenylium Derivative

摘要

The DNA binding affinity and anion effect on the aggregation of a G-quadruplex selective ligand,6a,12a-diazadibenzo-[a,g]fluorenylium derivative,were studied by UV-Vis absorption,molecular modeling and fluorescence spectroscopy.The anion character and its concentration influenced the spectral properties of ligand aggregates.Observed spectral peculiarities were discussed in terms of the formation of H- and J-type aggregates.The DNA binding mode and affinity of the ligand depended on the salt concentration;preferential binding to G-quadruplex DNA was clearly seen at higher salt concentration.