Assessment of Genetic Diversity of Biodiesel Species Pongamia pinnata Accessions using AFLP and Three Endonuclease-AFLP

摘要

Efficacy of two dominant molecular markers, namely, amplified fragment length polymorphism (AFLP) and three endonuclease (TE)-AFLP, were assessed in 20 individuals of the biodiesel species Pongamia pinnata. Four primer combinations generated a total of 254 and 194 bands in AFLP and TE-AFLP, respectively. Both techniques could unequivocally identify each accession used in this study. The Jaccard's similarity coefficient ranged from 0.30 to 0.90 for AFLP and from 0.25 to 0.85 for TE-AFLP. The correlation coefficient between AFLP and TE-AFLP dendrogram was 0.56 which was low but significant (P < 0.001). Values of effective multiplex ratio, marker index, and resolving power were markedly higher in AFLP than in TE-AFLP. However, the band intensities across different lanes were uniform in TE-AFLP leading to easy and accurate scoring of gels which resulted in slightly higher bootstrap values with TE-AFLP data as compared to AFLP data. Inferences based on TE-AFLP data had similar level of biological relevance as compared to AFLP data when location and diameter of trees were taken in to consideration. However, the easy scorability of TE-AFLP profiles is extremely important and especially desirable in studies requiring genotyping of large number of individuals distributed across many gels.