Expression of an oxalate oxidase gene in tomato and severity of disease caused by Botrytis cinerea and Sclerotinia sclerotiorum

摘要

A cDNA clone of wheat oxalate oxidase(OxO)under the control of the constitutive CAMV 35S promo tor was expressed in tomato plants by Agrobacterium-mediated transformation.Twenty-six transgenic tomato lines were obtained and analysed.PCR experiments confirmed the incorporation of the OxO gene in all tested tomato lines.The transgenic tomato plants expressed a 124-kDa protein showing OxO activity,and were able to convert different oxalic acid(OA)concentrations in vitro.In a detached leaf assay,most of the transgenic lines showed reduced disease symptoms compared with controls,following inoculation with Botrytis cinerea.In addition,leaves of the line T15 showed a marked reduction in symptoms compared with the control following inoculation with Sclerotinia sclerotiorum.