First report in Spain of a variant of Tomato mosaic virus (ToMV) overcoming the Tm-2~2 resistance gene in tomato (Lycopersicon esculentum)

摘要

A naturally infected tomato plant showing stunting and necrosis in the apical leaves was tested for viruses by ELISA using polyclonal antisera (Loewe).The sample tested positive for Tobacco mosaic virus (TMV) and Tomato mosaic virus (ToMV),and negative for Arabis mosaic virus,Cucumber mosaic virus,Pepino mosaic virus,Potato virus X,Potato virus Y,Tomato spotted wilt virus and Tomato bushy stunt virus.Leaf extracts were not infective by mechanical inoculation to Cucumis sativus,and induced necrotic local lesions on Datura stramonium,Petunia hybrida,Chenopodium quinoa,Nicotiana glutinosa,Nicotiana tabacum cv.Xanthi,and pepper (Capsicum annuum) cvs Pekin (Fito SA) and Oman (Seminis),which carry the L4 gene for resistance to Pepper mild mottle virus and TMV.