RAPD markers in diversity detection and variety identification of Tibetan hulless barley

摘要

RAPD markers were generated from 6 groups of Tibetan hulless barley (23 varieties): Lasagoumang, QB, Zangqing, Guoluo, Dongqing, and Hymalayia. Of the 48 fragments generated by 5 selected primers (among 68 primers), 44 appeared to be polymorphic (92%). Cluster analysis was performed (RAPDistance 1.04). The 23 varieties were divided into 2 groups, and the molecular foundation of genetic diversity was explored. In addition, to identify the varieties, one DNA fingerprint was constructed based on 17 bands amplified with S32 and 2 bands with S18. A specific RAPD fragment can be used to select for high and low beta-glucan content varieties.