Identification of Molecular Markers Associated with Semigamy in Cotton

摘要

Semigamy, controlled by one incomplete dominant gene (Se) in Pima cotton (Gossypium barbadense), is an aberrant form of sexual reproduction or apomixis, in which the sperm and egg nuclei fail to fuse and proceed to develop independently resulting in various outcomes, such as haploids, diploids, and chimeras. The objective of this study was to develop molecular markers linked to the semigamy gene using a (Pima S-1?×?57-4)?×?Pima S-1 BC1F1 mapping population consisting of 34 semigametic and 30 non-semigametic progenies. Random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), ATG-anchored AFLP (ATG-AFLP), sequenced tagged site (STS), and single-strand conformational polymorphism (SSCP) markers were tested. None of the apparent polymorphic RAPD, AFLP, and ATG-AFLP markers were linked to the semigamy gene, while three polymorphic markers developed from genes based on comparative microarray and differential display analyses, SSCP Se18-1800 and Se18-3000, and STS Se19, were mapped to a linkage group carrying the semigamy gene. The results present evidence that targeted gene mapping is an efficient method using markers developed from comparative gene expression studies. The current work represents the first study in identifying DNA markers associated with semigamy in plants, providing an incentive toward high-resolution mapping and isolation of the semigamy gene.