Comparison of the activities of CaMV 35S and FMV 34S promoter derivatives in Catharanthus roseus cells transiently and stably transformed by particle bombardment.

摘要

Activities of several cauliflower mosaic caulimovirus (CaMV) 35S and figwort mosaic caulimovirus (FMV) 34S promoter derivatives fused to the gusA [uidA] reporter gene were compared in suspension-cultured C. roseus cells that were transiently and stably transformed using particle bombardment. The 35S and a deletion derivative of the 34S promoter combined with particle bombardment conferred high levels of expression in cells. Enhancer multimerization did not have a positive effect on promoter activity in contrast to results obtained with other plant species, indicating that absolute and relative promoter activities can differ between plant species.