Identification of polymorphism by random amplified polymorphic DNA (RAPD) in groundnut (Arachis hypogaea L.)

摘要

Random amplified polymorphic DNA (RAPD) analysis with a single random primer, OPX-10 (5'-CCCTAGACTG-3') revealed distinct polymorphism among closely related groundnut (Arachis hypogaea) varieties. The primer appeared very specific to groundnut as it did not reveal polymorphism in other crops. Considering the narrow genetic base of groundnut in general and that of the varieties analysed, all having been derived from a single variety (Spanish Improved), OPX-10 primer appears to have amplified a fast evolving region of the genome.