Regeneration of plantlets through somatic embryogenesis from nucellus tissue of Citrus macroptera Mont. var. anammensis ('Sat Kara')

摘要

Somatic embryogenesis and plantlet regeneration were achieved in callus cultures of nucellus derived from undeveloped ovule of immature fruits of Citrus macroptera. Four types of media were used but only modified MS medium supplemented with malt responded well. Calli produced in malt supplemented MS medium were embryogenic in nature. After transfer of this embryogenic callus in hormone free MS medium, somatic embryos were developed. Independent plantlets were developed from these somatic embryos byfurther subculture in the same medium. After five weeks of culture the plantlets were transferred to pot soil mixed with cow-dung derived biogas slurry and survived well.