Silibinin in vitro protects A549 cells from staphylococcus aureus-mediated injury and in vivo alleviates the lung injury of staphylococcal pneumonia

摘要

In this study, hemolysis, Western blot, and real-time RT-PCR assays were performed to evaluate silibinins activity against S. aureus ??-toxin secretion. In addition, live/dead cell staining and lactate dehydrogenase activity assays were introduced to examine the influence of silibinin on ??-toxin-induced cell injury in human alveolar epithelial cells. Furthermore, we tested the influence of silibinin on S. aureus pneumonia in a mouse model. We show that silibinin inhibits the expression of ??-toxin in a dose-dependent manner and alleviates ??-toxin-induced lung cell injury. The IC 50 of silibinin that inhibits the hemolytic activity of S. aureus was 14.33 mg/mL for strain 8325-4. Moreover, this compound provides effective protection on the lung injury of staphylococcal pneumonia. ? Georg Thieme Verlag KG Stuttgart New York.