Evaluation of the open time of calciumchannels at variation potential generationin wheat leaf cells

摘要

The role of ions in the generation and mechanism of propagation of variation potential (VP) has been widelyinvestigated. It is likely that Ca2Cinflux via calcium channels is an initial stage of VP; however, development of long-term membrane depolarization requires prolonged open times of calcium channels. We investigated depolarizationtime in the present study. It was shown that local burning induced VP in wheat seedling and the electrical response wassuppressed under EGTA presence. Depolarization formation, which may indicate open time of calcium channels at VPgeneration, was observed up to 30 s after reaction induction when calcium ions were added to initially calcium-freemedium. Long-term calcium channel open time may be the reason for long membrane depolarization at VP and mayalso be connected with the type of channels participating in wound reaction propagation.