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首页> 外文期刊>Plant Science: An International Journal of Experimental Plant Biology >Genome-wide identification of novel internal control genes for normalization of gene expression during various stages of development in rice
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Genome-wide identification of novel internal control genes for normalization of gene expression during various stages of development in rice

机译:全基因组范围内的新型内部控制基因鉴定,用于水稻发育各个阶段基因表达的正常化

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摘要

An internal control gene with highly uniform expression throughout the experimental conditions is required for accurate and reliable gene expression results. However, no gene can serve as a universal internal control, implying the need for identification of most suitable internal control gene(s) for experimental conditions being analyzed. In an earlier study, we identified UBQ5 and eEF1-l as the most suitable internal control genes by validating the expression of ten housekeeping genes in rice. The aim of this study was to identify the most suitable novel internal control genes at the whole genome level for normalization of gene expression during various developmental stages in rice, the model crop plant. A systematic analysis of the whole genome microarray data for various stages of vegetative and reproductive development in rice was performed. Several novel internal control genes have been identified, which display highly uniform expression levels in all the developmental stages analyzed and outperform many traditional internal control genes. The expression of these genes ranges from low to very high, which make them suitable for normalization of gene expression over a wide range of transcript levels. The expression stability of the novel genes identified has also been validated by geNORM and NormFinder softwares. The novel genes with highly uniform expression identified in this study can be used for more accurate normalization of transcript levels during various developmental stages in rice and should enable the detection of biologically significant changes in gene expression.
机译:需要一个在整个实验条件下均具有高度均匀表达的内部对照基因,才能获得准确可靠的基因表达结果。但是,没有基因可以用作通用的内部对照,这意味着需要针对正在分析的实验条件鉴定最合适的内部对照基因。在较早的研究中,我们通过验证水稻中十个管家基因的表达,确定UBQ5和eEF1-1是最合适的内部控制基因。这项研究的目的是在全基因组水平上确定最合适的新型内部控制基因,以使模型作物水稻(水稻)各个发育阶段的基因表达正常化。对水稻营养和生殖发育各个阶段的全基因组微阵列数据进行了系统分析。已经鉴定出几种新颖的内部控制基因,它们在所分析的所有发育阶段均显示出高度均一的表达水平,并且优于许多传统的内部控制基因。这些基因的表达范围从低到非常高,这使得它们适合在广泛的转录水平上使基因表达正常化。鉴定出的新基因的表达稳定性也已通过geNORM和NormFinder软件进行了验证。在这项研究中鉴定出的具有高度均一表达的新基因可用于水稻不同发育阶段的转录水平更准确的标准化,并应能够检测基因表达的生物学显着变化。

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