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Enhanced photosynthesis rate in genetically engineered indica rice expressing pepc gene cloned from maize

机译:从玉米克隆的表达pepc基因的转基因in稻的光合作用速率提高

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Phosphoenolpyruvate carboxylase (pepc) gene is known to play the key role in C-4 photosynthesis by catalyzing initial fixation Of CO2 in C4 maize. We have introduced intact maize pepc gene in indica rice by biolistic transformation. Integration of the gene was confirmed by PCR and Southern analysis. RT-PCR and Northern blot showed expression of the gene in RNA level in transgenic plants. Immunoblot studies showed the presence of 106 kDa PEPC protein in transgenic plants. The transformants showed enhancement in photosynthesis rate in high temperature conditions. (C) 2007 Elsevier Ireland Ltd. All rights reserved.
机译:磷酸烯醇丙酮酸羧化酶(pepc)基因通过催化C4玉米中CO2的初始固定,在C-4光合作用中起关键作用。通过基因枪法转化,我们在in稻中引入了完整的玉米pepc基因。通过PCR和Southern分析证实了基因的整合。 RT-PCR和Northern印迹显示该基因在转基因植物中以RNA水平表达。免疫印迹研究表明在转基因植物中存在106 kDa PEPC蛋白。转化子在高温条件下显示出光合作用速率的增强。 (C)2007 Elsevier Ireland Ltd.保留所有权利。

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