Impact of microspore developmental stage on induction and plant regeneration in rye anther culture.

摘要

Rye anthers grouped into eight length classes, containing microspores from early uninucleate to binucleate developmental stage, were cultured to study the effect of microspore development on induction of embryogenesis and regeneration of green and albino plants. Three rye cultivars were monitored in two tests. Callus and embryoid induction increased in all cases beyond first pollen mitosis. Embryo quality was influenced by microspore age, and the ability of embryoids to develop into plants decreased as induction rate increased. Microspore developmental stage did not influence the proportion of albino plants of all regenerants. These findings have consequences for studies on regeneration and in determining the optimum microspore developmental stagefor efficient regeneration of green rye plants.