DEVELOPMENTAL MAPPING OF NODULATION EVENTS IN PEA (PISUM SATIVUM L) USING SUPERNODULATING PLANT GENOTYPES AND BACTERIAL VARIABILITY REVEALS BOTH PLANT AND RHIZOBIUM CONTROL OF NODULATION REGULATION

摘要

Pea (Pisum sativum L.) cv. Frisson and its two supernodulating mutants P64 and P88 were inoculated with two different Rhizobium strains and nodule development was followed using a cytological clearing method. Supernodulating plant mutants were characterized by a faster appearance of nodules and a more extensive nodule distribution over the root system. General characteristics of nodule development were similar to the wild-type Frisson. The formation of nodule meristem was identified as a limiting stage of nodule development arrest. The microsymbiont was found to have an effect on the meristem activity of nodules, increasing nodule dry matter accumulation and branching. [References: 42]