Nucleotide Sequence of a cDNA Clone Encoding 1 -Aminocyclopropane-1 -Carboxylic Acid Synthase from Broccoli

摘要

Ethylene regulates many processes in plant growth, development, and senescence. Two enzymes, ACC synthase and ACC oxidase, are responsible for ethylene biosynthesis from its precursor S-adenosyl Met (Yang and Hoffman, 1984), and blocking the synthesisof these enzymes in an-tisense RNA experiments results in reduced ethylene synthesis (Hamilton et al., 1990; Oeller et al., 1991). Functional identification of cDNA clones encoding ACC synthase has been achieved by expression studies in Escherichia coli and yeast and by sequence comparisons to peptides purified from ACC synthase protein (Sato and Theologis, 1989; Yip et al., 1990; Dong et al., 1991).