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首页> 外文期刊>Plant physiology >DIFFERENTIAL EXPRESSION OF LIPOXYGENASE ISOENZYMES IN EMBRYOS OF GERMINATING BARLEY
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DIFFERENTIAL EXPRESSION OF LIPOXYGENASE ISOENZYMES IN EMBRYOS OF GERMINATING BARLEY

机译:大麦胚乳中脂氧合酶同工酶的差异表达

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摘要

Expression of lipoxygenase was studied in barley (Hordeum distichum L.) embryos during germination. Total lipoxygenase activity was high in quiescent grains, dropped during the Ist d of germination, and subsequently increased to a level similar to that in quiescent grains. The contribution of two isoenzymes, lipoxygenases 1 (LOX-1) and 2 (LOX-2), was studied at the activity, protein, and mRNA levels. Activity ratios of the two isoforms were determined via the ratio of 9- and 13-hydroperoxides, which are formed from linoleic acid. Isoenzyme protein levels were determined using specific monoclonal antibodies. mRNA levels were studied using the specific cDNA probes LoxA and LoxC, which correspond to LOX-1 and LOX-2, respectively. The major difference in temporal expression of LOX-1 and LOX-2 was observed in quiescent grains. At this stage, LOX-1 contributed almost exclusively to total lipoxygenase activity. LOX-2 activity rapidly increased until d 2 of germination. From this time point onward, LOX-1 and LOX-2 showed similar patterns at both activity and protein levels. The tissue distribution of the two isoenzymes in the germinating embryo was closely similar, with the highest expression levels in leaves and roots. The levels of LOX-1 and LOX-2 may be regulated mainly pretranslationally, as suggested by the similarity of the protein and mRNA patterns corresponding to the two isoforms. [References: 30]
机译:研究了大麦(Hordeum distichum L.)胚发芽过程中脂氧合酶的表达。静态谷粒中的总脂氧合酶活性较高,在萌发的第一天下降,随后增加到与静态谷粒相似的水平。在活性,蛋白质和mRNA水平研究了两种同功酶脂氧合酶1(LOX-1)和2(LOX-2)的贡献。通过由亚油酸形成的9-和13-氢过氧化物的比率确定两种同工型的活性比率。使用特异性单克隆抗体测定同工酶蛋白水平。使用特定的cDNA探针LoxA和LoxC研究mRNA的水平,分别对应于LOX-1和LOX-2。在静止的谷粒中观察到LOX-1和LOX-2的时间表达的主要差异。在此阶段,LOX-1几乎完全贡献了总脂氧合酶活性。 LOX-2活性迅速增加直到发芽的d 2为止。从这个时间点开始,LOX-1和LOX-2在活性和蛋白质水平上都显示出相似的模式。两种同工酶在发芽胚中的组织分布非常相似,在叶和根中的表达水平最高。 LOX-1和LOX-2的水平可能主要在翻译前受到调节,这是由对应于两种同工型的蛋白质和mRNA模式的相似性所暗示的。 [参考:30]

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