Thirty-nine potato cultivars and advanced breeding lines were evaluated at the Russell E. Larson Agricultural Research Center in Rock Springs, PA. The soil type was a Hagerstown silty clay loam. The previous crop was wheat. Entries were planted on 20May in a randomized complete block design with three replicates. Plots consisted of a single row 4-ft long with five seed pieces planted in each plot with a 4-ft break between plots. Each entry had an adjacent row of the susceptible cv. Dark Red Norlandas a spreader row. Fertilization was 1015 lb/A of 10-10-10 (N-P-K) banded in row at planting. Precipitation was 5.71, 3.84, 5.52, and 1.38 in. for Jun, Jul, Aug, and Sep, respectively. On 23 Jul, spreader rows were spray-inoculated with a conidial mixture of two isolates of Alternaria solani, at a concentration of 4.77 x 104 conidia/ml, to promote uniform spread of the pathogen to all treatment plots. For each plot, the percentage of diseased foliage was visually assessed on 4, 11, 17, 22 and 28 Aug. Disease data were expressed as the area under the disease progress curve (AUDPC), subjected to an analysis of variance and means separated using Fisher's protected least significant difference test (SAS v. 9.4, SAS Institute, Cary, NC).
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机译：在宾夕法尼亚州Rock Springs的Russell E. Larson农业研究中心对39个马铃薯品种和高级育种系进行了评估。土壤类型是黑格斯敦粉质粘土壤土。以前的作物是小麦。 5月20日以随机完整区组设计（三份重复）种植了参赛作品。地块由4英尺长的单行组成，每个地块中种有5个种子，地块间的间隔为4英尺。每个条目都有易感简历的相邻行。深红色Norlandas吊具行。播种时施肥为1015 lb / A的10-10-10（N-P-K）。 6月，7月，8月和9月的降雨量分别为5.71、3.84、5.52和1.38英寸。 7月23日，对撒布机行分别喷洒两种分离的链霉菌链孢菌的分生孢子混合物，浓度为4.77 x 104分生孢子/ ml，以促进病原体向所有处理区的均匀传播。对于每个样地，在8月4日，11日，17日，22日和28日通过视觉评估患病叶子的百分比。疾病数据表示为疾病进展曲线（AUDPC）下的面积，经过方差分析并使用Fisher的受保护的最小显着差异检验（SAS v。9.4，SAS Institute，Cary，NC）。
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