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Evaluation of registered and experimental fungicides for control of Rhizoctonia root rot of zinnia, 2014

机译:评价用于控制百日草根瘤菌根腐病的注册和实验杀菌剂,2014年

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Inoculum was prepared by growing R. solani on potato dextrose agar for 4 wk. Flasks filled with two parts millet and one part water were sterilized. Four 1.5-in. plugs of the infested agar were then placed into each flask. The infested millet was allowed to grow for three weeks before being mixed (8 oz/1 ft3) into a soilless media (Suremix, MI Grower Products Inc, Galesburg MI). Six-week-old healthy zinnia 'Exquisite Pink' seedlings were transplanted into 4-in. pots containing the infested soilless media on 16 Jun. Fungicides were applied as a drench in sufficient volume to displace 10% of the water (ca. 3 fl oz/pot) on 17 Jun. Six plants per treatment were placed into a completely randomized design. Plants were watered as needed and fertilized three times weekly with 200 ppm of Peters 20-20-20 water soluble fertilizer (The Scotts Company, Marysville, OH). Greenhouses temperatures averaged 81°F during the day and 71°F at night. A plant health rating and the percentage of dead plants were assessedon 23 Jun and 3 Jul.
机译:通过在马铃薯右旋糖琼脂上生长solani solani 4周来制备接种物。将装有两份小米和一份水的烧瓶进行灭菌。四个1.5英寸然后将感染的琼脂的塞子放入每个烧瓶中。将受侵染的小米生长三周,然后将其混合(8盎司/ 1平方英尺)到无土的培养基(Suremix,MI Grower Products Inc,Galesburg MI)中。将六周龄的健康百日草“精致粉红色”幼苗移植到4英寸长的植物中。 6月16日,将盆栽盛满受感染的无土培养基。在6月17日,以足够量的杀菌剂施用,以置换10%的水(约3 fl oz /盆)。每次处理将六株植物置于完全随机的设计中。根据需要给植物浇水,并每周用200 ppm的Peters 20-20-20水溶性肥料(俄亥俄州马里斯维尔的斯科茨公司)施肥3次。日间温室平均温度为81°F,夜间平均温度为71°F。在6月23日和7月3日评估了植物健康等级和死植物百分比。

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