On 22 Oct 09, bare-root transplants from Canada were set into 50:50 methyl bromide:chloropicrin fumigated soil in plastic-mulched, raised beds. The beds were 28-in. wide on 4-ft centers and contained two staggered rows of plants spaced 15 in. apart within rows and 12 in. between rows. To provide inoculum, ‘Sweet Charlie’ transplants naturally infected by X. fragariae were set at regular intervals between rows in each bed, and later removed when the disease became established. Transplants were irrigated by overhead sprinklers for 10 days to aid establishment, then irrigated and fertilized through drip tape. Treatments were arranged in a randomized complete block design with four blocks, each in a separate adjacent bed. Before applications were initiated, plants were removed to create individual plots in a staggered arrangement. Each plot contained 12 plants in 8.1 ft of bed. Experimental products were applied with a CO2 backpack sprayer delivering 100 gal/A at 50 psi through two TeeJet disc-core hollow cone nozzles spaced 12 in. apart on the boom. Applications were made from 2 Dec to 10 Mar on a 7-day schedule (15 applications), a 14-day schedule (8 applications), or before predicted rain/freeze events and/or after actual rain/freeze events (variable timing and number of applications). To suppress fungal diseases, Captan 80WDG was applied weekly with a tractor-mounted hydraulic sprayer in 100 gal/A at 200 psi (1.5 lb/A in Dec and early Jan; 3.0 lb/A in Feb and early Mar). Fruit were harvested twice weekly from 5 Jan to 16 Mar (20 harvests). Marketable fruit were counted and weighed to determine yield. Eight plants in each plot were individually evaluated for ALS severity on 9 Feb and 18 - 24 Mar. In Feb, leaves that were killed or >75% blightedby ALS (dead leaves) were counted and removed from evaluated plants. In Mar, dead leaves, partially blighted leaflets, and healthy leaves were enumerated. The number of partially blighted leaves was determined by dividing the number of partially blighted leaflets by three since each leaf has three leaflets. Individual plant assessments were averaged for each plot. Data were analyzed by two-way ANOVA (P = 0.05).
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机译：2009年10月22日，将来自加拿大的裸根移栽物置于塑料覆盖的高架床中50:50的甲基溴：氯仿熏蒸土壤中。床是28英寸。宽4英尺中心，包含两排交错的植物，每排之间间隔15英寸，两排之间间隔12英寸。为了提供接种物，将自然感染了草莓角斑病杆菌的“甜蜜查理”移植物固定放置在每张床的行之间，并在疾病确定后将其移除。用高架喷头灌溉移植物10天以帮助建立，然后通过滴灌带灌溉并施肥。治疗以随机完整区组设计安排，每个区有四个区，每个区都位于单独的相邻床中。在启动应用程序之前，将植物移走以交错排列的方式创建单个地块。每个地块在8.1英尺的床位上包含12株植物。实验产品使用CO2背包喷雾器进行喷涂，该喷雾器通过两个在吊臂上间隔12英寸的TeeJet盘芯空心锥形喷嘴以50 psi的压力提供100 gal / A的流量。申请时间为12月2日至3月10日，为期7天（15个申请），14天（8个申请），或者在预计的降雨/冻结事件之前和/或在实际的降雨/冻结事件之后（可变时间和申请数量）。为抑制真菌疾病，每周以200 psi的压力（100 gal / A）用拖拉机安装的液压喷雾器施用Captan 80WDG（12月和1月初为1.5 lb / A; 2月和3月初为3.0 lb / A）。从1月5日至3月16日，每周两次收获水果（20次收获）。对可出售的水果进行计数并称重以确定产量。在2月9日和3月18日至24日分别评估了每块地中的8棵植物的ALS严重程度。2月份，对被杀死的叶片或被ALS枯萎的叶片（死叶）计数超过75％进行计数，并将其从评估的植物中移除。在3月，列举了枯叶，部分枯萎的小叶和健康的叶子。通过将部分枯萎的小叶的数量除以三，可以确定部分枯萎的叶的数量，因为每片叶子都有三张小叶。对每个样地的单个工厂评估值取平均值。数据通过双向方差分析进行分析（P = 0.05）。
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