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首页> 外文期刊>Plant Disease >Infectivity and Inoculum Production of Phytophthora ramorum on Roots of Eastern United States Oak Species
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Infectivity and Inoculum Production of Phytophthora ramorum on Roots of Eastern United States Oak Species

机译:美国东部橡树种根上疫霉疫霉的感染性和接种量

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摘要

Little is known about colonization of roots of trees by Phytophthora ramorum. We examined zoospore concentration and exposure time needed to infect six Quercus (oak) species and the inoculum produced from their roots. Sprouted acorns, exposed to zoospores (3,000/ml) for different times and transplanted to potting soil, were susceptible to infection within 1 h of exposure but root weights were not impacted after 4 weeks (P = 0.952). Roots of Quercus prinus seedlings, inoculated with sporangia, had 0.6 to 3.2% colonization of the total root mass after 5 months. Neither root lesions nor obvious root sloughing were observed. Inoculum threshold levels were tested by exposing radicles to varying zoospore concentrations for 24 h. Results showed that radicle infection occurred even at 1 zoospore/ml. To test inoculum production, roots were inoculated with sporangia and transplanted into pots. Periodically, samples of runoff were collected and plated on selective medium. Afterward, root segments were plated to calculate percent colonization. After 16 and 35 days, root colonization and inoculum production from oak was lower than that of Viburnum tinus, a positive control. This study shows that P. ramorum is able to infect sprouted oak acorns and produce secondary inoculum, which may be important epidemiologically.
机译:疫霉菌对树木根部的定植知之甚少。我们检查了感染六个栎属(橡树)物种及其根部的接种物所需的游动孢子浓度和暴露时间。发芽的橡子在不同的时间暴露于游动孢子(3,000 / ml)并移植到盆栽土壤中,在暴露后1小时内很容易受到感染,但4周后根重没有受到影响(P = 0.952)。接种孢子囊的5个月后,栎类幼苗的根部定植了总根质量的0.6%至3.2%。既没有观察到根部病变,也没有观察到明显的根脱落。通过将胚根暴露于变化的游动孢子浓度24小时来测试接种量阈值水平。结果表明,即使以1个游动孢子/ ml发生了胚根感染。为了测试接种量,将根部接种孢子囊并移植到盆中。定期收集径流样品并将其铺在选择性培养基上。之后,将根段铺平以计算定殖百分比。 16天和35天后,橡树的根部定植和接种量低于阳性对照荚莲(Viburnum tinus)。这项研究表明,P。ramorum能够感染发芽的橡子橡子并产生次生接种物,这可能在流行病学上很重要。

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