First report of crown rot of grafted cucumber caused by Fusarium solani in China.

摘要

In March 2009, a severe crown rot causing yellowing and wilting of the leaves was observed on grafted cucumber in a large number of greenhouses in Lingyuan, western Liaoning Province in China. Symptoms consisted of dark brown, water-soaked lesions, and a dense, white mycelial mat at the base of the stem. In 30 surveyed greenhouses in Sanshijiazi Village in the city of Lingyuan, the incidence of affected plants ranged from 10 to 40%, which caused serious economic losses. Fusarium spp. were isolated from the surface-sterilized basal stems of symptomatic plants on potato dextrose agar. Based on morphological characteristics, the fungus was identified as F. solani [Haematonectria haematococca]. For confirmation, the internal transcribed spacer region of ribosomal DNA was amplified and sequenced. A 449-bp sequence shared 99% homology with that of a F. solani GenBank accession previously reported from Japan (No. AF150473.1). This is thought to be the first report of crown rot of grafted cucumber caused by F. solani in China.Digital Object Identifier http://dx.doi.org/10.1094/PDIS-03-10-0217