First report of spot form of net blotch of barley caused by Pyrenophora teres f. maculata in Hungary.

摘要

A countrywide survey of fungal diseases of barley (Hordeum vulgare) was conducted from 2005 to 2009 in Hungary. Unusual leaf necrosis varying in shape from 1.0 x 2.0 mm necrotic flecks to 15.0 x 20.0 mm ovoid spots was observed. Sometimes, a chlorotic halo surrounding the dead area was observed. Lesions appeared on various cultivars in many commercial fields and experimental plots at a number of sampling sites. Symptomatic leaves were taken to the laboratory and incubated in a moist chamber at room temperature to induce sporulation of the pathogen. Conidiophores on the diseased tissues were single or in small groups, dark brown, and bore several hyaline-to-olive brown, almost cylindrical conidia with 3-7 pseudosepta. Species identity was confirmed by PCR assays with specific primers developed for the barley pathogenic Pyrenophora species. Of the 169 isolates, 41 were identified as P. teres f. maculata, the spot form of the net blotch pathogen. The remaining isolates were either P. graminea or P. teres f. teres, the leaf stripe and net form of net blotch pathogens of barley, respectively. This is thought to be the first report of the occurrence of P. teres f. maculata in Hungary.