Plantlet production from the male inflorescence tips of Musa acuminata cultivars from South India.

摘要

Inflorescence apices are suitable explants for the rapid in vitro propagation of Musa spp. However, the diploid and triploid banana cultivars showed different in vitro responses with respect to the hormone combinations in Murashige and Skoog medium. The diploid cultivar (Sannachenkadali, AA) induced a maximum number of multiple shoots in 8.9 micro M 6-benzyl adenine (BA) whereas the triploid cultivar (Red banana, AAA) exhibited maximum multiplication in 22.2 micro M 6-benzyl adenine. MS medium supplemented with 11.4 micro M indole acetic acid and 17.8 micro M BA was also suitable for shoot proliferation in triploid cultivar but not in the diploid cultivar. The regenerated shoots were rooted in Murashige and Skoog basal medium within 10-15 days. The rooted plantlets were transferred to vermiculite and maintained at a temperature of 25+or-2 degrees C for 10 days and then at room temperature (30-32 degrees C) for 2 weeks before transferring to potted soil compost mixture. The plantlets showed 100% survival..