Isolation and functional characterization of a novel rice constitutive promoter

摘要

A novel rice constitutive promoter (P (OsCon1) ) was isolated. The molecular mechanism of the promoter activity was investigated. P (OsCon1) could be used as an alternative constitutive promoter for crop transgenic engineering. Monocot constitutive promoter is an important resource for crop transgenic engineering. In this report, we isolated a novel promoter, Oscon1 promoter (P (OsCon1) ), from the 5' upstream region of a constitutively expressed rice gene OsDHAR1. In P (OsCon1) ::GUS transgenic rice, we showed that P (OsCon1) had a broad expression spectrum in all tested tissues. The expression of the promoter was further analyzed in comparison with the previously characterized strong constitutive promoters. P (OsCon1) exhibited comparable activity to OsCc1, OsAct1 or ZmUbi promoters in most tissues, and more active than 35S promoter in roots, seeds, and calli. Further quantitative assays indicated that P (OsCon1) activity was not affected by developmental stages or by environmental factors. Further, 5'-deletions analysis indicated that the distinct regions might contribute to the strong expression of P (OsCon1) in different tissues. Overall, our results suggest that P (OsCon1) is a novel constitutive promoter, which could potentially use in transgenic crop development