Comprehensive selection of reference genes for quantitative gene expression analysis during seed development in Brassica napus

Machado Ronei Dorneles; Christoff Ana Paula; Loss-Morais Guilherme; Margis-Pinheiro Marcia; Margis Rogerio; Koerbes Ana Paula

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Comprehensive selection of reference genes for quantitative gene expression analysis during seed development in Brassica napus

机译：甘蓝型油菜种子发育过程中定量基因表达分析的参考基因的综合选择

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MicroRNAs have higher expression stability than protein-coding genes in B. napus seeds and are therefore good reference genes for miRNA and mRNA RT-qPCR analysis.

机译：在油菜种子中，MicroRNA具有比蛋白质编码基因更高的表达稳定性，因此是miRNA和mRNA RT-qPCR分析的良好参考基因。

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来源
《Plant Cell Reports》 |2015年第7期|共11页
作者
Machado Ronei Dorneles; Christoff Ana Paula; Loss-Morais Guilherme; Margis-Pinheiro Marcia; Margis Rogerio; Koerbes Ana Paula;
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正文语种 eng
中图分类植物细胞学;
关键词
RT-qPCR; microRNAs; geNorm; Normalization; Rapeseed; Seeds;

机译：RT-qPCR;microRNA;geNorm;标准化;油菜籽;种子;

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专利

1. Comprehensive selection of reference genes for quantitative gene expression analysis during seed development in Brassica napus [J] . Machado Ronei Dorneles, Christoff Ana Paula, Loss-Morais Guilherme, Plant Cell Reports . 2015,第7期

机译：甘蓝型油菜种子发育过程中定量基因表达分析的参考基因的综合选择
2. Analysis of the differential expression of the genes related to Brassica napus seed development [J] . Huang Jin-Yong, Jie Zhi-Jing, Wang Li-Jun, Molecular biology reports . 2011,第2期

机译：甘蓝型油菜种子发育相关基因的差异表达分析
3. Selection and evaluation of novel reference genes for quantitative reverse transcription PCR (qRT-PCR) based on genome and transcriptome data in Brassica napus L. [J] . YangH., LiuJ., HuangS., Gene: An International Journal Focusing on Gene Cloning and Gene Structure and Function . 2014,第1期

机译：基于甘蓝型油菜的基因组和转录组数据，选择和评估用于定量逆转录PCR（qRT-PCR）的新型参考基因。
4. Serial Analysis of Gene Expression (SAGE) applied to the study of Brassica napus seed development [C] . Christian Obermeier, Bashir Hosseini, Rod Snowdon Proceedings of the 12th International Rapeseed Congress: Sustainable Development in Cruciferous Oilseed Crops Production . 2007

机译：基因表达的序列分析（SAGE）应用于甘蓝型油菜种子发育的研究
5. Genetic characterization of exotic germplasm in spring-type Brassica napus L.: Validation, confirmation, tester complementation and prediction of hybrid seed yield quantitative trait loci. [D] . Kramer, Chad Curtis. 2007

机译：春季型甘蓝型油菜外来种质的遗传特征：杂种种子产量数量性状基因座的验证，确认，测试者互补和预测。
6. Selection and validation of reference genes for quantitative gene expression analyses in various tissues and seeds at different developmental stages in Bixa orellana L. [O] . Viviane S. Moreira, Virgínia L. F. Soares, Raner J. S. Silva, 2018

机译：Bixa orellana L.在不同发育阶段的各种组织和种子中定量基因表达分析的参考基因的选择和验证。
7. Genome-wide survey of flavonoid biosynthesis genes and gene expression analysis between black- and yellow-seeded Brassica napus [O] . Cunmin Qu, Huiyan Zhao, Fuyou Fu, 2016

机译：黑色和黄色种子甘蓝型油菜黄酮类生物合成基因的全基因组调查和基因表达分析

Comprehensive selection of reference genes for quantitative gene expression analysis during seed development in Brassica napus

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