Evaluating sugarcane (Saccharum sp.) cultivars for water deficit tolerance using some key physiological markers

摘要

Seven sugarcane (Saccharum sp.) commercial cultivars, viz., UT-94-2-483, LK92-11, K84-200, K97-32, K95-84, K88-92 and K 92-80, originally derived from meristem cuttings were subjected to simulated osmotic stress (as 200 mM mannitol) under controlled environmental conditions. Proline content in the leaf tissues of all cultivars except K92-80, increased in plants subjected to mannitol-induced osmotic stress. Chlorophyll a (Chl_a), chlorophyll b (Chi_b,), total chlorophyll (TC), maximum quantum yield ofPSII (F_v/F_m), and photon yield of PSII (PHI_(PSII)) of all seven cultivars decreased under osmotic stress resulting in a reduction in net-photosynthetic rate (P_n). A positive correlation was found between Fv/ F_m and PHI_(PSII), proline content and non-photochemical quenching (NPQ), PHI_(PSII) and P_n, and P_n and plant dry weight in the sugarcane cultivars. Based upon Ward's multivariate cluster analyses of data for proline content, photosynthetic capacity, chlorophyll fluorescence, and growth inhibition, three cultivars (K88-92, K92-80 and UT-94-2-483) were identified as water deficit sensitive, whereas four (K84-200, K95-84, K97-32 and LK92-11) as water deficit tolerant. These observations on different cultivars sensitivity/tolerance were confirmed by growth and yield attributes measured in a field trial. The plant dry weight (in vitro) correlated positively with total stalk weight of sugarcane cultivars