cDNA cloning and characterization of a novel gene differentially expressed in developing seeds of high-oleate safflower(carthamus tinctorius L.)

摘要

Transcriptomes of the developing seeds of high-oleate and high-linoleate strains of safflower(Carthamus tinctorius) were compared using a fluorescent differential display technique. Two cDNA fragments(O1-1 and O2-3) were identified as the bands originated from the genes highly or specifically expressed in the developing seeds of the high-oleate safflower. A cDNA clone, CTOS-1, encoding a novel protein with an isoprenoid binding site at the C-terminus was isolated by screening a cDNA library constructed from the developing seeds of high-oleate safflower using the O2-3 fragment as a probe. Northern blot analysis indicated that CTOS-1 gene expresses only in the developing seeds of the high-oleate safflower. The transcrip cannot be detected either in the developing seeds of the high-linoleate safflower nor in the leaves, stems and flower buds of the two strans. Southern blot analysis using the CTOS-1 cDNA as a probe indicated the presence of restriction fragment length polymorphism between the high-oleate and high-linoleate strains.