Somatic embryo formation and plant regeneration in 'Zaoh' line No.2 of Japanese angelica tree (Aralia elata seem.)

摘要

Mass propagation of the Japanese angelica tree (Aralia elata seem.)'Zaoh' line No.2 was established through somatic embryos. Petioles of leaflets were cultured for induction of calli on an MS medium containing 1 mg l~(-1) of 2,4- D in combination with 0.5 mg l~(-1) BA. The initiated calli were moved onto MS medium supplemented with glutaminc 450 mg l~(-1) and asparagine 300 mg l~(-1). An embryogenic callus developed after 5 months and was moved onto regulator- free MS medium. Numerous plantlets were regenerated from this embryogenetic callus, and rooted plantleis were potted after acclimation and planted to the field. The plants had the same characteristics in morphology in the field, except for the number of thorns per internode. The plants alsohad the same resistance to Phytophthora disease as was in the original genotype 'Zaoh' line No.2. This study provided a method for mass propagation from petioles of leaflets and proves that regenerated plants maintain the morphogenctic characteristics anddisease resistance of the original genotype.