Molecular and cytogenetic characterization of triploid somatic hybrids between 'Shogun' mandarin and grapefruit.

摘要

Somatic hybrids were produced between embryogenic callus protoplasts of 'Shogun' mandarin (Citrus reticulata Blanco, 2n = 2x = 18) and leaf protoplasts of grapefruit (C. paradisi Macf., 2n = 2x = 18) by eiectrofusion. Hybridity of the two regeneratedplants was confirmed by leaf characteristics and nuclear genome analysis using RAPD and PCR-RFLP. Flow cylomctry and root-tip cell count of the two hybrids revealed a chromosome number of 27. These triploid somatic hybrids on transfer to field showed normal morphology and vigorous growth. Field performances as well as disease resistance arc under investigation.