Induction of morphologically changed petals from mutagen-treated apical buds of rose and plant regeneration from varied petal-derived calli

摘要

The apical buds of lateral branches asexually multiplied by cutting were treated with some chemical mutagens, and the growth and differentiation or morphological changes of the mutagen-treated buds were traced in developed flowers. As a result, thevariations in size, shape, color and number of petals were detected most frequently in the flowers that were developed from apical buds treated with N-methyl-N'-nitro-N-nitrosoguanidine at 100 #mu#g ml~(-1). The variant petals were cultured on MS mediumsupplemented with NAA and BAP for in vitro isolation and multiplication of morphologically altered rose plants. Embryogenic calli were obtained via adventitious roots induced from the petals and successfully differentiated to intact plants. Consequently,the regenerated plants produced the varied flower different from that originally used for tissue culture. Thus, the present study suggested that our approach would provide an effective method for easily and rapidly inducing variations in flowers of roseand for in vitro multiplication of their regenerants.