An efficient plant regeneration through embryogenic callus formation and direct somatic embryogenesis via immature embryo culture in Ipomoea purpurea and I-tricolor

摘要

We established an efficient plant regeneration system to form embryogenic calli in common morning glory ( Ipomoea purpurea) and blue morning glory ( I. tricolor). Immature embryos of both morning glories cultured on media containing 1 mg l(-1) 4-fluorophenoxyacetic acid (4FA) and 6% sucrose formed many embryogenic calli. The frequency of embryogenic callus formation was highest in I. purpurea strain Q74 (42.5%) and in I. tricolor cultivar 'Flying Saucers' (36.7%). Embryogenic callus formation differed with the genotypes in both morning glories. Numerous somatic embryos were formed from the embryogenic calli when the calli were transferred onto plant growth regulator-free medium