Microgravity affects the photosynthetic apparatus of Brassica rapa L.

摘要

Structural and functional characteristics of the photosynthetic apparatus in 15-day old Brassica rapa plants grown aboard the space shuttle Columbia (STS-87) have been studied. Maintaining of the same growth conditions for control plants was realized using the Orbiter Environmental Simulator in Kennedy Space Center. The main differences in spaceflight plants in comparison with control ones have been shown to be the following. An average volume of one mesophyll palisade cell increased approximately twice and the chloroplast number per cell by 69.8%. Partial volumes of stromal thylakoids, starch grains and plastoglobuli also increased by 19.4%, 20.6% and 2 times accordingly. At the same time, the grana number per chloroplast decreased. Greater diversity of the thylakoid length in grana and a decrease in thylakoid membrane stacking were revealed. A decrease of PSII and PSI light-harvesting antennae has been detected, for PSII by an increase of Chl a/b ratio and kinetics delay in chlorophyll fluorescence induction, and for PSI by a decrease of integral intensity in the excitation spectrum of fluorescence at 735 nm, which indicated a decline of PSI absorption cross-section. Some distortion of PSI complexes have been displayed by fluorescence spectra. A slight decrease in PSII photochemistry yield was detected for the spaceflight material. PSI is concluded to be more susceptible to the microgravity conditions.