Phenotypic and molecular dissection of ICRISAT mini core collection of peanut (Arachis hypogaea L.) for high oleic acid.

摘要

High oleic acid (O) and low linoleic acid (L) make peanut oil ideal for longer storage and better human health. Among the ICRISAT mini core collection accessions, oleic acid ranged from 33.60% to 73.54%. Accessions belonging to ssp. hypogaea had higher oleic acid (54.16%) compared with those of ssp. fastigiata (45.70%). An O:L ratio up to 6.93 was found among ssp. hypogaea. Additional varieties, mutants, germplasm lines and breeding lines had oleic acid within the range of mini core accessions. Mutations in ahFAD2A, which along with its homologous gene, ahFAD2B code for delta-12-desaturase, resulted in higher oleic acid and O:L ratio. ahFAD2A mutant allele was found in 49.5% of the accessions, and its frequency was higher in ssp. hypogaea (84.52%) than in ssp. fastigiata (19.39%). ahFAD2A mutation had a maximum contribution of 18.82, 12.98 and 10.52 towards the phenotypic variance of O, L and O:L ratio, respectively. Genotypes with high oleic acid levels could not reveal "A" insertion mutation in ahFAD2B. Accessions with high oleic acid could be employed for improving peanut oil quality. copyright 2012 Blackwell Verlag GmbH.